Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA NAPQI Wikipedia Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Metabolic activation pathway of acetaminophen. Generally, NAPQI is Download Scientific Diagram Can Tylenol (acetaminophen) deplete glutathione levels in the body?