Can Tylenol (acetaminophen) deplete glutathione levels in the body? Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA PGC 1 Induces Susceptibility To Acetaminophen Driven Acute Liver Failure Scientific Reports Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.